at the University of Toronto
2024
Knox, J., Burns, A.R., Cooke, B., Cammalleri, S.R., Kitner, M., Ching, J., Castelli, J.M.P., Puumala, E., Snider, J., Kourey, E., Collins, J.B., Geissah, S., Dowling, J., Andersen, E.C., Stagljar, I., Cowen, L.E., Zasada, I., and Roy, P.J. 2024. Cyprocide Selectively Kills Nematodes via Cytochrome P450 Bioactivation. Nature Communications, 15:5529. Read the paper here.
Kamal, M.*, Knox, J.*, Horne, R.I., Tiwari, O.S., Burns, A.,R., Han, D., Bar-Yosef, D.L., Gazit, E., Vendruscolo, M., Roy, P.J. 2024. A Rapid in vivo Pipeline to Identify Small Molecule Inhibitors of Amyloid Aggregation. Nature Communications, 15:8311. Read the paper here.
Au, A., Giuliani, M., Harrington, S., Roy, P.J., and Yip, C.M. Low-cost line-scan imaging for real-time phenotypic screening of C. elegans. In Review.
2023
Burns, A.R.§, Ross, R., Kitner, M., Knox, J., Cooke, B., Volpatti, J., Vaidya, A. S., Puumala, E., Palmeira, B.M., Redman, E.M., Snider, J., Marwah, S., Chung, S., MacDonald, M.H., Tiefenbach, J., Hu, C., Xiao, Q., Finney, C.A.M., Krause, H.M., MacParland, S.A., Stagljar, I., Gilleard, J.S., Cowen, L.E., Meyer, S., Cutler, S.R., Dowling, J.J., Lautens, M., Zasada, I., Roy, P.J§. 2023. Selective Control of Parasitic Nematodes Using Bioactivated Nematicides. Nature, 618, pages102–109. Read the paper here.
Harrington S.*, Pyche J.*, Burns A.R., Spalholz T., Ryan, K.T., Baker RJ, Ching J, Rufener, L., Lautens M., Kulke D., Vernudachi, A., Zamanian, M., Deuther-Conrad W., Brust P., and Roy P.J. 2023. Nemacol is a Small Molecule Inhibitor of C. elegans Vesicular Acetylcholine Transporter with Anthelmintic Potential. Nature Communications 14(1816). Read the paper here.
Kamal, M., Tokmakjian, L., Knox, J., Han, D., Moshiri, H., Magomedova, L., Nguyen, K.C.Q., Zheng, H., Burns, A.R., Cooke, B., Lacoste, J., Yeo, M., Hall, D.H., Cummins, C.L., and Roy, P.J. 2023. PGP-14 Establishes a Polar Lipid Permeability Barrier within the C. elegans Pharyngeal Cuticle. PLoS Genetics. Nov 6;19(11):e1011008.
doi: 10.1371/journal.pgen.1011008. Read the paper here.
2022
Harrington, S., Knox, J., Burns, A.R., Loon-Choo, K., Au, A., Kitner, M., Haeberli, C., Pyche, J., D’Amata, C., Kim, H-Y., Volpatti, J.R., Guiliani, M., Snider, J., Wong, V., Palmeira, B.M., Redman, E.M., Vaidya, A.S., Gilleard, J., Stagljar, I., Cutler, S.R., Kulke, D., Dowling, J.J., Yip, C.M., Keiser, J., Zasada, I., Lautens, M., and Roy, P.J. 2022. Egg-Laying and Locomotory Screens with C. elegans Yield a Nematode-Selective Small Molecule Stimulator of Neurotransmitter Release. Communications Biology, 2022. 5, 865. Read the paper here.
Kamal, M.*, Tokmakjian, L.*, Knox, J.*, Mastrangelo, P., Ji, J., Cai, H., Wojciechowski, J.W., Hughes, M.P., Takács, K., Chu, X., Pei, J., Grolmusz, V., Kotulska, M., Forman-Kay, J.D., and Roy, P.J. 2022. A Spatiotemporal Reconstruction of the C. elegans Pharyngeal Cuticle Reveals a Structure Rich in Phase-Separating Proteins. ELife. Oct 19;11:e79396. doi: 10.7554/eLife.79396. Read the paper here.
Roy, PJ. 2022. Temporal Regulation of Gene Expression in Post-Mitotic Cells is Revealed from a Synchronized Population of C. elegans Larvae. MicroPubl Biol. Jun 10;2022:10.17912/micropub.biology.000587. Read the paper here.
Murareanu, B.M., Antao, N.V., Zhao, W., Dubuffet, A., El Alaoui, H., Knox, J., Ekiert, D.C., Bhabha, G., Roy, P.J., and A. W. Reinke. 2022. High-throughput small molecule screen identifies inhibitors of microsporidia invasion and proliferation in C. elegans. Nature Communications 13(1):5653. doi: 10.1038/s41467-022-33400-y. Read the paper here.
Loll-Krippleber, R., Sajtovich, V.A., Ferguson, M.W., Ho, B., Burns, A.R., Payliss, B.J., Bellissimo, J., Peters, S., Roy, P.J., Wyatt, H.D.M., and Brown, G.W. 2022. Development of a yeast whole-cell biocatalyst for MHET conversion into terephthalic acid and ethylene glycol. Microbial Cell Factories 21, 280. https://doi.org/10.1186/s12934-022-02007-9. Read the paper here.
Cammalleri, S. R., Knox, J., and Roy, P.J§. 2022. Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci. J. Vis. Exp. (179), e63438, doi:10.3791/63438. Read the paper here.
2021
Knox, J., Joly. N., Linossi, E. M., Carmona-Negrón, J.A., Jura, N., Pintard, L., Zuercher, W., and Roy, P.J. 2021. A Survey of the Kinome Pharmacopeia Reveals Multiple Scaffolds and Targets for the Development of Novel Anthelmintics. Scientific Reports. 11:9161 doi.org/10.1038/s41598-021-88150-6. Read the paper here.
2020
Volpatti, J., Endo, Y., Knox, J., Groom, L., Brennan, S., Noche, R., Noche, R., Zuercher, W., Roy, P., Dirksen, R. T., and Dowling, J.J. 2020. Identification of drug modifiers for RYR1 related myopathy using a multi-species discovery pipeline. eLife 2020;9: e57481 DOI: 10.7554/eLife.57481. Read the paper here.
2019
Kamal, M., Moshiri, H., Magomedova, L., Han, D., Nguyen, K.C.Q., Yeo, M., Knox, J., Bagg, R., Won, A.M., Szlapa, K., Yip, C., Cummins, C.L., Hall, D.H., and Roy, P.J. 2019. The Marginal Cells of the Caenorhabditis elegans Pharynx Scavenge Cholesterol and Other Hydrophobic Small Molecules. Nature Communications. 10, 1-16. Read the paper here.
2018
Otten, C., Knox, J., Boulday, G., Eymery, M., Haniszewski, M., Neuenschwander,M., Radetzki, S., Vogt, I, Hähn, T., De Luca, C., Cardoso, C., Hamad, S., Gil, C., I., Roy, P., Albiges-Rizo, C., Faurobert, C., von Kries, J.P., Campillos, M., Tournier-Lasserve, E., Derry, W.B., and Abdelilah-Seyfried, S. 2018. Systematic pharmacological screens uncover novel pathways involved in cerebral cavernous malformations. EMBO Mol Medicine, 10(10). pii: e9155. doi: 10.15252/emmm.201809155. Read the paper here.
2017
Burns, A.R., Bagg, R., Yeo, M., Luciani, G.M., Schertzberg, M., Fraser, A.G., and Roy, P.J. 2017. The Novel Nematicide wact-86 Interacts with Aldicarb to Kill Nematodes. PLoS Neglected Tropical Diseases. 11(4): e0005502. Read the paper here.
2016
D’Souza, S.A., Rajendran, L., Bagg, R., Barbier, L., and Roy, P.J. 2016. The MADD-3 LAMMER Kinase Interacts with a p38 MAP Kinase Pathway to Regulate the Display of the EVA-1 Guidance Receptor in Caenorhabditis elegans. PLoS Genetics. 12(4): e1006010. Read the paper here.
2015
Burns, A.R.*, Luciani, G.M., Musso, G., Bagg, R., Yeo, M., Zhang, Y., Rajendran, L., Glavin, J., Hunter, R., Redman, E., Stasiuk, S., Schertzberg, M., McQuibban, G.A., Caffrey, C.R., Cutler, S., R., Tyers, M., Giaever, G., Nislow, C., Fraser, A.G., MacRae, C. A., Gilleard, J. and Roy, P.J. 2015. Caenorhabditis elegans is a useful model for Anthelmintic Discovery. Nature Communications. 6:7485 | DOI: 10.1038/ncomms8485. Read the paper here.
Chan, K.*, Seetharaman, A.*, Selman, G.*, and Roy, P.J. 2015. Immunoprecipitation of Proteins in Caenorhabditis elegans. Bioprotocol 5(7). Read the paper here.
2014
Chan, K.*, Seetharaman, A.*, Bagg, R., Selman, G., Zhang, Y., Kim, J., and Roy, P.J. 2014. EVA-1 Functions as an UNC-40 Co-Receptor to Enhance Attraction to the MADD-4 Guidance Cue in Caenorhabditis elegans. PLoS Genetics 10(8):e1004521. doi: 10.1371/journal.pgen.1004521. Read the paper here.
2013
Morf, M.K., Rimann, I, Alexander, M., Roy, P.J., and Hajnal, A. 2013. The Caenorhabditis elegans homolog of the Opitz syndrome gene, madd-2/Mid1, regulates anchor cell invasion. Developmental Biology, 374, 108-114. Read the paper here.
2012
Burns, A., and Roy, P. 2012. To Kill a Mocking Worm: Strategies to Improve Caenorhabditis elegans as a Model System for use in Anthelmintic Discovery. A chapter in the book, “Parasitic Helminths: Targets, Drugs and Vaccines”. Eds. Caffrey, C. , Selzer, P., Wiley VCH, Publishers. Read the chapter here.
Tharmalingam, S., Burns, A. R., Roy, P.J., and Hampson, D.R. 2012. Orthosteric and Allosteric Drug Binding Sites in the C. elegans mgl-2 Metabotropic Glutamate Receptor. Neuropharmacology 63, p667-674. Read the paper here.
2011
Luciani, G.M., Magomedova, L., Puckrin, R., Urbanus, M., L., Wallace, I.M., Giaever, G., Nislow, C., Cummins, C.L., and Roy, P.J. 2011. Dafadine Inhibits DAF-9 to Promote Dauer-Formation and Longevity of Caenorhabditis elegans. Nature Chemical Biology 7, 891-893. doi: 10.1038/nchembio.698. Read the paper here.
Seetharaman, A., Selman, G., Puckrin, R., Barbier, L., Wong, E., D’Souza, S.A., and Roy, P.J. 2011. MADD-4 is a Secreted Cue Required for Midline-Oriented Guidance in Caenorhabditis elegans. Developmental Cell 21, p669-680.
Wallace, I.M., Urbanus, M.L., Luciani, G.M., Arora, K., Heisler, L.E., Lee, W., Proctor, M., Burns, A.R., St. Onge, R.P., Roy, P.J., Bader, G.D., Nislow, C., and Giaever, G. 2011. Compound prioritization methods increase rates of chemical probe discovery in model organisms. Chem Biol. 18, p1273-1283. Read the paper here.
2010
Burns, A.R., Wallace, I.M., Wildenhain, J., Tyers, M., Giaever, G., Bader, G., Nislow, C., Cutler, S.R., and Roy, P.J. 2010. A Predictive Model for Drug Bioaccumulation and Bioactivity in Caenorhabditis elegans. Nature Chemical Biology, 6, p549-557 (doi: 10.1038/nchembio.380). Read the paper here.
Alexander, M., Selman, G., Seetharaman, A., Chan, K.K., D’Souza, S.A., Byrne, A.B., and Roy, P.J. 2010. MADD-2, a Homologue of the Opitz Syndrome Protein MID1, Regulates Guidance to the Midline through UNC-40 in Caenorhabditis elegans. Developmental Cell, 18, p961–972. Read the paper here.
2009
Alexander, M.*, Chan, K.K.*, Byrne, A.*, Selman, G., Lee, T., Ono, J., Wong, E., Puckrin, R., Dixon, S.J., and Roy, P.J. 2009. An UNC-40 Pathway that Directs Postsynaptic Membrane Extension in Caenorhabditis elegans. Development, 136, 911-922. Read the paper here.
Hui, K.*, Kwok, T.C.Y.*, Kostelecki, W., Leen, J., Roy, P.J., and Feng, Z.P. 2009. Differential sensitivities of CaV1.2 IIS5-S6 mutants to 1,4-dihydropyridine analogs. European Journal of Pharmacology, 602, p255-261. Read the paper here.
Wang, W.§, Sun, Y., Dixon, S., Alexander, M., and Roy, P. 2009. An Automated Micropositioning System for Investigating C. elegans Locomotive Behavior. JALA. 14 (5), p269-276. doi:10.1016/j.jala.2008.12.006. Read the paper here.
2008
Dixon, S.J.*, Alexander, M.*, Chan, K.K., Roy, P.J. 2008. Insulin-like Signaling Negatively Regulates Muscle Arm Extension through DAF-12 in Caenorhabditis elegans. Developmental Biology, 318, p153-161. Read the paper here.
Kwok, T.C.Y.*, Hui, K.*, Kostelecki, W., Selman, G., Ricker, N., Feng, Z.P., and Roy, P.J. 2008. A Screen for Dihydropyridine (DHP)-Resistant Worms Reveals a Mechanism for DHP-Blockage of Mammalian Calcium Channels. PLoS Genetics, 4(5):e1000067. Read the paper here.
Arnoldo, A., Curak, J., Kittanakom, S., Chevelev, I., Lee, V.T., Sahebol-Amri, M., Koscik, B., Ljuma, L., Roy, P.J., Bedalov, A., Giaever, G., Nislow, C., Merrill, R. A., Lory, S., and Stagljar, I. 2008. Identification of Small Molecule Inhibitors of Pseudomonas aeruginosa Exoenzyme S Using a Yeast Phenotypic Screen. PLoS Genetics, 4(2): e1000005. Read the paper here.
2007
Von Stetina, S. E., Watson, J.D., Fox, R.M., Olszewski, K.L., Roy, P.J., Miller, D.M. III. 2007. Cell-specific microarray profiling experiments reveal a comprehensive picture of gene expression in the C. elegans nervous system. Genome Biology, 8, R135. Read the paper here.
Byrne, A., Weirauch, M., Wong, V., Koeva, M., Dixon, S.J., Stuart, J., and Roy, P.J. 2007. A Global Analysis of Genetic Interactions in Caenorhabditis elegans. Journal of Biology, 6(3):8. Read the paper here.
2006
Kwok, T., Ricker, N., Fraser, R., Chan, A., Burns, A., Stanley, E.F., McCourt, P., Cutler, S., and Roy, P.J. 2006. A Small Molecule Screen in C. elegans Yields a New Calcium Channel Antagonist. Nature. 441, 91-95. Read the paper here.
Dixon, S.J., Alexander, M., Ricker, N., Fernandes, R., Roy, P.J. 2006. FGF Signaling Negatively Regulates Muscle Membrane Extension in C. elegans. Development.133, 1263-1275. Read the paper here.
Burns, A.R., Kwok, T.C.Y., Howard, A., Houston, E., Johanson, K., Chan, A., Cutler, S.R., McCourt, P., Roy, P.J. 2006. High-throughput Screening of Small Molecules for Bioactivity and Target Identification in Caenorhabditis elegans. Nature Protocols. 1, 1906-1914. Read the paper here.
2005
Dixon, S.J., and Roy, P.J. 2005. Muscle arm development in Caenorhabditis elegans. Development. 132, 3079-3092. Read the paper here.